Figure 2.

Generation and analysis of temperature-sensitive plp2 alleles. (A) Wild-type yeast (PLP2), or two temperature-sensitive alleles of PLP2, namely, plp2-1 and plp2-2, were spotted on YPD media and grown at the temperatures shown for 48 h. plp2-1 displays a more severe ts phenotype than plp2-2. (B) Reversibility of the plp2-ts alleles was assessed after growth at 37°C for the times shown. Shifted cells were plated at a permissive temperature and the number of microcolony forming cells was assessed under a microdissection microscope. plp2-2 temperature sensitivity is largely reversible compared with the more severe plp2-1 allele.