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Receptor-mediated endothelial cell O2.− generation results in a stable increase of nuclear HE fluorescence. HPMVECs were loaded with HE (10 μM) and stimulated with Ang II (2 μM) or thrombin (0.5 U/ml) for 1 h with or without pretreatment with the NADPH oxidase inhibitor apocynin (Apo; 2 μM) or the anion channel blocker DIDS (300 μM). Nuclear HE fluorescence was quantitated from the confocal microscopic images. Data represent mean ± SE of five independent fields (n = 3).
