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. 2000 May 23;97(12):6896–6901. doi: 10.1073/pnas.120170197

Figure 4.

Figure 4

SOS1 is similar to Na+/H+ antiporters. (A) Alignment of SOS1 (accession number AF256224) with Na+/H+ antiporters NHE1 from Chinese hamster (P48761) and NhaP from Pseudomonas aeruginosa (BAA31695). The sequences were aligned by the program clustalw (http://dot.imgen.bcm.tmc.edu:9331/multialign/Options/clustalw.html). Amino acids identical in at least two proteins are highlighted in black, and conservative substitutions are highlighted in gray. Asterisks indicate conserved residues that were substituted in sos1 mutant alleles. (B) Phylogenetic analysis of SOS1 and other representative Na+/H+ antiporters. Multiple sequence alignment was performed with clustalw. The alignment is based on the N-terminal 450 amino acids of SOS1. Evolutionary distances were calculated by the neighbor joining method, and the phylogenetic tree was drawn by the program drawgram (http://bioweb.pasteur.fr/seqanal/phylogeny/phylip-uk.html). The accession numbers and sources of each of the other representative Na+/H+ antiporters are as follows: NHE1 (P19634), Homo sapiens; NHE2 (AAD41635), H. sapiens; NHE3 (P48764), H. sapiens; NHE4 (P26434), Rattus norvegicus; NHE5 (AAC98696.1), H. sapiens; NHE6 (NP_006350), H. sapiens; NHA1 (NP_013239), S. cerevisiae; NHX1 (NP_010744), S. cerevisiae; AtNHX1 (AAD16946.1), A. thaliana; SOD2 (CAA77796.1), S. pombe; NhaA (P13738), Escherichia coli; and NhaP (BAA31695.1), P. aeruginosa.