Figure 2.

Deduced amino acid sequence of the glucose acyltransferase (AT) and its alignment with barley serine carboxypeptidase I (BI). The numbers on the right are the residue numbers for the last amino acid on each line. Straight lines above the AT sequence indicate peptide sequences obtained from the 34-kDa and 24-kDa subunits. PT sequences are tryptic peptide sequences from the 34-kDa subunit. N24 is the N terminus of the 24-kDa subunit. ●, The R or K N-terminal to tryptic cleavage sites. Between AT and BI, colons are used to link the identical sequences, and dots are used to link the similar sequences. *, The active catalytic Ser-158, His-446, and Asp-393 sites in BI. @, The sites corresponding to those involved in the binding of substrate C-terminal carboxylate group in yeast serine carboxypeptidase.