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. 2007 Jun 1;21(11):1367–1381. doi: 10.1101/gad.1545107

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Figure 2. — Allelic loss of beclin1 promotes DNA damage response under metabolic stress. Representative beclin1^+/+ (WB-A2) and beclin1^+/− (BLNB-A4) iBMK cell lines expressing Bcl-2 were subjected to ischemia stress and the DNA damage response was measured by the induction of γ-H2AX by immunofluorescence and Western blotting. (A) Representative photomicrographs showing accumulation of γ-H2AX in the beclin1^+/− iBMK cell line expressing Bcl-2 (BLNB-A4) as a marker of DNA damage. (B) Quantitation of γ-H2AX-positive cells in beclin1^+/+ (WB-A2) and beclin1^+/− (BLNB-A4) iBMK cell lines expressing Bcl-2 in A. The percentage of nuclei with positive γ-H2AX foci was tabulated. Data represent mean ± SD. Note enhanced accumulation of γ-H2AX in the beclin1^+/− iBMK cell line expressing Bcl-2 (BLNB-A4) compared with the wild type. (C) Western blot time course showing γ-H2AX (marker of DNA damage) and GRP-78 (marker of UPR) in beclin1^+/+ (WB-A2) and beclin1^+/− (BLNB-A4) iBMK cell lines, both expressing Bcl-2.