Figure 4.

Inhibition of NFκB decreases RANTES gene expression in response to U-STAT3. (A) hTERT-HME1-derived cells were transfected transiently with the pcDNA3.1-mIκBα construct, which encodes the NFκB superrepressor and, 48 h later, total RNAs were isolated and analyzed. (B) The RANTES promoter-driven luciferase reporter construct pGL2-220 was transfected with pCH110, with or without pcDNA3.1-mIκBα, into hTERT-HME1-derived cells and, 48 h later, luciferase assays were performed. (C) hTERT-HME1-derived cells were transfected transiently with a siRNA directed against p65 and, 24 h later, the cells were transfected again as in A. The cells were harvested after 48 h more and total RNA was extracted. All of the mRNAs shown were assayed on the same Northern transfer.