Table 1.

Promoters that bind to USTAT3 have κB sites

A ChIP assay was performed with chromatin from YF cells (containing the Y705F mutant of STAT3) by using anti-Flag M2. The immunoprecipitated DNA was amplified by PCR and cloned, and 12 clones were sequenced. Five of the cloned fragments had no promoter activity in a luciferase reporter assay (data not shown). The seven fragments that did have promoter activity are shown. Five of these were from genes previously shown to be responsive to Y705F-STAT3 and U-STAT3 and two were not analyzed previously (N/A). The fold induction of each of these five genes in response to high-level expression of Y705F-STAT3 is shown, as are the positions of the κB elements and consensus sites for other transcription factors.