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AHL-dependent DNA binding of purified (His)6ExpR to the Cy3-labeled UpsinI wild-type fragment and the UpsinI fragment carrying point mutations. The mutated UpsinI fragment contains three altered bps at positions −106 (T replaced by G), −104 (T replaced by G), and −99 (G replaced by C) upstream of the sinI start codon. Lanes 1 and 2, UpsinI only; lanes 3 and 4, UpsinI with (His)6ExpR; lanes 5 and 6, UpsinI with (His)6ExpR and oxo-C14-HL.
