TABLE 2.
Summary of binding capacity of various ERα (liganded or unliganded) measured at two different ERE packing densities
| DNA packing density
|
||||
|---|---|---|---|---|
| Sample | 0.78 ERE/SA ratio (mol/mol) or 5.0 (pmol/cm2) | 0.23 ERE/SA ratio (mol/mol) or 1.5 (pmol/cm2) | ||
| ERα binding capacity (pmol/cm2) | ERα/ERE (mol/mol) | ERα binding capacity (pmol/cm2) | ERα/ERE (mol/mol) | |
| Ctrl ERα | 9.8 ± 1.3 | 2.0 ± 0.3 | 5.8 ± 0.6 | 3.9 ± 0.4 |
| E2-ERα | 6.2 ± 0.7 | 1.2 ± 0.1 | 5.3 ± 1.0 | 3.5 ± 0.7 |
| 4OHT-ERα | 3.2 ± 0.4 | 0.6 ± 0.1 | 2.4 ± 0.7 | 1.6 ± 0.5 |
*ERα binding capacity (pmol/cm2) or ERα per DNA ratio (mol/mol) are calculated from the angle shift values using an AutoLab SPR sensitivity of 833 ng/(cm2/deg) and the molecular mass of 21.4, 66.4, 66.7, and 66.8 kDa for the 34-bp ERE, Ctrl ERα, E2-ERα, and 4OHT-ERα, respectively. Values are mean ± SD obtained from three to five experiments.