Figure 1.

β-synemin and α-dystrobrevin interact in vivo and co-localize in rat muscle. (A) Using an anti-α-dystrobrevin antibody, α-dystrobrevin and its interacting proteins were immunoprecipitated from rat protein muscle extracts. Western blot analysis was performed to localize the β-synemin protein. β-synemin was found to be present in the muscle extract (1) and immunoprecipitated pellet (2), while it was not detected in the supernatant (3). (B) Using an anti-β-synemin antibody, β-synemin and its interacting proteins were immunoprecipitated from rat protein muscle extracts. Western blot analysis was performed to localize the α-dystrobrevin isoforms. Both α-dystrobrevin-1 and -2 were detected in the muscle extract (1) and pellet (2), while only α-dystrobrevin-2 was present in the supernatant (3). (C) Rat tibialis anterior muscle was sectioned 21 days following cardiotoxin injection and double immunostained with antibodies against α-dystrobrevin antibody (left panel), β-synemin (center panel), and the merged image (right panel). Bar = 50 μm