Figure 4.

lin-35 mutants affect cyclin expression and intestinal nuclear ploidy. (A) qRT-PCR was performed on wild-type and lin-35(rr33) L1 larvae and the levels of cyclin expression were determined compared to wild-type. The bars represent 4 independent qRT-PCR reactions from RNA isolated from wild type and lin-35(rr33) animals. A positive value indicates an increase in gene expression compared to wild-type, while a negative value indicates a relative decrease in gene expression. The standard deviation is derived from the 4 independent trials. (B) DNA quantification was performed on the intestinal nuclei of both mutant and wild-type young adult hermaphrodites stained with propidium iodide. Each diamond represents a lin-35(r33) mutant intestinal nucleus and each square represents an individual wild type intestinal nucleus.