Fig. 6.

Short-term BSO exposure mimics the effect of AtPCS1 gene disruption in cad1-3 with respect to Cd²⁺ accumulation in roots and shoots. Four-week-old cad1-3, Adh/cad1-3 (Adh::TaPCS1/cad1-3), 35S/cad1-3 (35S::TaPCS1/cad1-3), and WT plants grown in hydroponic solution were pretreated with 0 mM/1 mM BSO for 12 h; 20 μM CdCl₂ was then applied to either the untreated plants (A) or the 1 mM BSO pretreated plants with 0.5 mM BSO for 54 h (B). Cd²⁺ accumulation in stems (S), rosette leaves (L), and roots (R) was determined by ICP-AES. Data show mean values ± SE, n = 6 and 3 plants in A and B, respectively.