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. 2007 May 25;3(5):e78. doi: 10.1371/journal.pgen.0030078

Figure 8. Quantitative RT-PCR of p21/WAF/CIP in Various Morphants.

Figure 8

p21 RNA levels were significantly increased in MO-injected embryos that showed neural death (Smo MO, Wnt5 MO1, and Mdm2 MO) as compared to the uninjected control (UI). Interestingly, p21 levels were not significantly altered by Wnt5 MO2 (which does not induce neural death). Very importantly, p21 levels were significantly reduced in the respective MO-injected embryos upon p53 co-knockdown (Mdm2 MO p < 0.01, Wnt5 MO2 p < 0.01, Smo MO p < 0.002; t-test p values for unpaired sets), strongly suggesting that p21 is transcriptionally upregulated by p53 in the morphants with neural death. Error bars indicate standard deviation.