Skip to main content
. 2007 May;9(5):435–442. doi: 10.1593/neo.07223

Figure 1.

Figure 1

shRNA, S-TRAIL, and Fluc lentiviral vectors. (A) A self-inactivating lentiviral system based on HIV-1 (CS-CG) [36] was used to construct vectors (i) expressing shRNA against human Bcl-2 and GFP under HI RNA polymerase III promoter and DsRed2 under theCMV promoter; and (ii) expressing S-TRAIL or Fluc under the CMV promoter with an IRES sequence followed by GFP and LacZ, respectively. (B) Human glioma, Gli36-EGFRvIII, U251, and U87 cells transduced in culture with LV-shBcl-2 and 293T cells transduced with LV-S-TRAIL at an MOI of 1 were visualized for DsRed2 or GFP fluorescence. Original magnification, ×10 (B).