Fig. 2.

Two types of aberrant alternative splicing between exons 6 and 7 of Foxn1^tw mRNA. (Top) Normal splicing between exons 6 and 7 of Foxn1 mRNA. The nucleotide numbers of 5′ and 3′ of intron 6 are 39651 and 43956, respectively. The nucleotide numbers are referred to in the reported M. musculus whn gene sequence, GenBank accession no. Y12488. (Middle) The major form of the mutant mRNA. This type, 77% of the total of Foxn1^tw mRNA, has a 54-bp deletion 5′ in exon 7 (43957–44010), generating an 18-aa deletion in the center of the DNA binding domain. (Bottom) The minor form of the mutant mRNA. This type, 23% of the total of Foxn1^tw mRNA, has a 44-bp cryptic exon (40541–40584) and a 34-bp deletion 5′ in exon 7 (43957–43991), generating a translational termination (43992–43994).