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. 2000 Oct 24;97(23):12446–12450. doi: 10.1073/pnas.220425597

Figure 2.

Figure 2

Interaction of CHOP-10 with C/EBPβ early in the differentiation program. Nuclear extracts were prepared from 3T3-L1 preadipocytes before (day 0) and at 4 and 28 h after induction of differentiation and subjected to immunoprecipitation with rabbit anti-C/EBPβ N-terminal antibody by using protein A-Sepharose. After three washes, supernatants and precipitates were separated by SDS/PAGE and immunoblotted with mouse anti-C/EBPβ C-terminal and mouse anti-CHOP-10 antibodies. Ab, antibody; Pi, preimmune serum; KD, kDa; and Hrs, hours after induction of differentiation.