Figure 5.

Effect of ALLN on the turnover of CHOP-10 protein and on CHOP-10 mRNA level. (A) 3T3-L1 preadipocytes were induced to differentiate as in Fig. 1 in the absence or presence of ALLN (26 μM) with or without 10 μg/ml cycloheximide (CHX). Nuclear extracts were prepared and immunoblotted with anti-CHOP-10 antibody at the indicated times (Hrs) after induction of differentiation. (B) After induction of differentiation in the absence or presence of ALLN (26 μM), total RNA was isolated and subjected (10 μg) to Northern analysis with CHOP-10 cDNA as a probe.