Table 1.
New 3′ end sequences in satC progeny generated in vivo from transcripts with or without 3′ end deletions
| Construct | Plant | 3′ end sequence | Construct | Plant | 3′ end sequence |
|---|---|---|---|---|---|
| WT | [GGACGGGGGGCCUGCCC] | CΔ4 | [GGACGGGGGGCCU] | ||
| 1 & 2 | GGACGGGGGGCCUGCCC (8, 7) | 1 & 2 | GGACGGGGGGCCUGCCC (7, 5) | ||
| 2 | 3′Δ18 (1) | 1 | GGACGGGGGGCCUGCCCU (1) | ||
| GGACGGGG (1) | |||||
| WT+8b | [GGACGGGGGGCCUGCCCggguaccg] | 2 | GGACGGGGGGCCUGCCCG (1) | ||
| 1, 2, 3 | GGACGGGGGGCCUGCCC (6, 11, 4) | GGACGGGGGGCCUUU (1) | |||
| 1 & 3 | GGACGGGG (1, 2) | ||||
| 1 | GGACGGGGGGCCUU (1) | CΔ4+8b | [GGACGGGGGGCCUggguaccg] | ||
| 3 | GGACGGGGGGCCUUU (1) | 1 & 2 | GGACGGGGGGCCUGCCC (4, 3) | ||
| GGACGGGGGGCCUGC (1) | GGACGGGGGGCCUGCCCU (2, 1) | ||||
| ∗GGACGGGGGGCCUggg (1, 3) | |||||
| CΔ3 | [GGACGGGGGGCCUG] | 1 | GACGGGG (1) | ||
| 1, 2, 3 | GGACGGGGGGCCUGCCC (10, 4, 4) | 2 | GGACGGGGGGCCUUU (1) | ||
| 1 | GGACGGGGGGCCUGCCCU (1) | TCV/satC recombinants: | |||
| 3 | GGACGGGGGGCCU (1) | 2 | GCGCGGGGGGGGGGCCUGCCCG (1) | ||
| CΔ3+8b | [GGACGGGGGGCCUGggguaccg] | ||||
| 1, 2, 3 | GGACGGGGGGCCUGCCC (1, 1, 1) | CΔ5 | [GGACGGGGGGCC] | ||
| 2 | GGACGGGGGGCCUG (1) | 1 & 2 | GGACGGGGGGCCGGCCC (3, 9) | ||
| GGACGGG (1) | 1 | GGACGGGGGGCCCGCCC (1) | |||
| 3 | GGACGGGGGGCCCUGCCC (1) | ∗GGACGGGGGGCGGGCCC (1) | |||
| ∗GGACGGGGGGCCCAGCCC (1) | ∗GGACGGGGGGCCGUUU (1) | ||||
| GGACGGGG (3) | GGACGGGG (2) | ||||
| TCV/satC recombinants: | GGACGG (1) | ||||
| 1 & 2 | GCGCGGGGGGGGCCUGCCCG (1, 1) | 2 | GGACGGGGGGCGGCCC (1) | ||
| 2 & 3 | GCGCGGGGGGGGGGCCUGCCCG (3, 1) | ||||
| GCGCGGGGGGGGCCUGCCC (4, 4) | CΔ5+8b | [GGACGGGGGGCCggguaccg] | |||
| 1 | GCGCGGGGGGGGGGCCUGCCC (6) | 1 & 2 | GGACGGGGGGCCUGCCC (4, 3) | ||
| GCGCGGGGGGGGGCCUGCCCG (1) | 1 | GGACGGGGGGCCUGGG (4) | |||
| GCGCGGGGGGGGGCCUGCCC (1) | 2 | GGACGGGGGGCCUGCCCU (1) | |||
| GCGGGGGGGGGCCUGCCC (1) | GGACGGGGGGCCUG (2) | ||||
| GCGCGG (1) | GGACGGG (1) | ||||
| 2 | GCGCGGGGGGGGCCUGCCCC (1) | TCV/satC recombinants: | |||
| 3 | GCGCGGGGGGGGGGCCUGCCC (1) | 1 & 2 | GCGCGGGGGGGGGGCCUGCCCG (1, 1) | ||
| GCGCGGGGGGGGCCUGCUU (1) | 2 | GCGCGGGGGGGGCCUGCCC (1) |
Sequences from position 340 to the 3′ end of satC transcripts used for inoculations are enclosed in brackets. Sequences identical to all or portions of the 3′ terminal motif (CCUGCCC-3′) are in bold. Lowercase letters represent plasmid-derived bases. Bases in italics denote the repaired 3′ end sequences of progeny satC-like species. Number of clones of each sequence recovered from individual plants is in parentheses. All clones terminated with poly(A) added during cloning, so 3′ end nontemplate additions of adenylates are not discernible. ∗ denotes sequences reinoculated to plants (progeny described in Table 2). WT, wild-type satC.