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. 2003 Jul 30;100(17):9768–9773. doi: 10.1073/pnas.1333958100

Fig. 6.

Fig. 6.

Interaction of MTMR6, MTMR7, and MTMR9. (A) Myc-tagged MTM proteins were expressed in COS-7 cells. Cell lysates were mixed with GSTMTMR7-SID-coil or GST-MTMR9-SID-coil protein beads, and a GST pull-down assay was performed. Mock, empty pCMV5-Myc vector is transfected; G, GST; C, coiled-coil domain. The numbers depicted under the diagrams of GST proteins indicate the amino acid numbers of each construct. (B) MTMR6 associates with full-length MTMR9. HA-tagged MTMR6 and MTMR7 were transfected into COS-7 cells with Myc-tagged MTMR9. HA-tagged proteins were immunoprecipitated (IP). Immunoprecipitates were analyzed by Western blotting with anti-Myc antibody. (C) Identification of MTMR7-binding domain of MTMR9. Myc-tagged MTMR7 was expressed in COS-7 cells. Cell lysates were mixed with GST-MTMR9-SID or GST-MTMR9-coil protein bound to glutathione-Sepharose 4B beads, and a GST pull-down assay was performed.