Table 1. Proteins locating to nucleocapsid and unknown localization.
| ORF | Identity | Library screen | SDS/PAGE, % coverage | MUDPIT-MS/MS | Western blot | Ref. |
|---|---|---|---|---|---|---|
| Identified by using multiple techniques | ||||||
| 9 | p78/83 | PKTAPETSTIVEVPTVLPK: LPPPAPSLSNVLSELK; SSTTNLIADVLADTINR | 34 | |||
| 49 | PCNA | 35 | ||||
| 54 | vp1054 | 36 | ||||
| 61 | FP25K | 8.8 | 37 | |||
| 65 | DNA polymerase | 8.6 | NDTQCANNTYKFCLYKMK | |||
| 66 | 93 kDa | 43.9* | LRQEFEIK; DENAERLSEIQLQK; SKLNTQLDELNSLFVK; QSVSIKDQEIAM; ESIADQAVKLLEQNQTDFESISEFISRDPAFNR | |||
| 67 | lef3 | X | ||||
| 70 | Hcf-1 | 1 | KENKEIYITSNK | |||
| 77 | Vlf-1 | 1 | 2.4* | 32 | ||
| 89 | p39 | 54.6 | RAVAPEYLQIDTEELR; RILIPSATNYQDVFNLNSM; NVLKFEGDTQR; VLPIFDEDDNQFK; NDFIPR; YTEGFTSTTQR; AVAPEYLQIDTEELR; FFDVTNAR; VIHSVYATTK; GGAGDQLFNNYSGFLQNLIR | 38 | ||
| 95 | Helicase | KLENVV | X | |||
| 100 | p6.9 | 37.5 | SSTGTTYGSTRR | 39 | ||
| 101 | BV/ODV-C42 | TTLEELLIER | 40 | |||
| 102 | 13.1 kDa | 23.6* | ILSTQSVGAR: LQTINTAASQTAASLLINDITPNKTESLK | |||
| 104 | p87 | 48.2 | SAEDDLLPTR | 41 | ||
| 109 | 44.8 kDa | 34.5* | IIFPYQLVPNVIIK; TNINFVTQR; FFAFPHNLVEPQSDVGNK; QAQSLLGIPDYSQTVVDFVK; PDFSPPNTFDYSDYANR | |||
| 114 | 49 kDa | ETIVNIINSYHNACQNLK; LAALDFIILM; NYVQPAIVNLFESHNR; IINELLFLNDNVNYATNK | ||||
| 129 | p24 | 42 | ||||
| 132 | 25.1 kDa | 41.1 | AIAAEQTLR | |||
| 142 | 49 kDa | 58.3* | GLPLFK; DLNPWVQNTLLK; NAFYAPK; ILSILAVNR; SKYTVVNSTK; YDHESSYIFYSK; LTGDVYVVDKNEK; YIKPGTPVYATNLFTSNPR; SGGGNLLTLERDHFK | |||
| 144 | ODV-EC27 | 2 | TYELAEFDLK; TVTEIVNSDEKIQK | 43 | ||
| 147 | IE1 | X | ||||
| Identified by using one technique | ||||||
| 5 | 12.4 kDa | ITEYVGDVK; INNAPVVASQHDYDRDQIKR | ||||
| 14 | lef1 | 2 | ||||
| 22 | 44 kDa | 29.6 | ||||
| 30 | 54.5 kDa | 1 | ||||
| 39 | p43 | 8.5 | ||||
| 58 | 6.8 | SKKFPIGEVVSTR | ||||
| 59 | 8.2 kDa | LFVETFTK | ||||
| 74 | 30.5 kDa | 1 | ||||
| 79 | 12.1 kDa | 2 | ||||
| 86 | PNK/PNL | 6.3 | ||||
| 88 | cg30 | VESLHFNVYSVNRNVVDVIK | ||||
| 92 | p33 | IPLTPLFSR | ||||
| 114 | 49 kDa | ETIVNIINSYHNACQNLK; LAALDFIILM; NYVQPAIVNLFESHNR; IINELLFLNDNVNYATNK | ||||
| 133 | Alk-exo | 1.8%* | ||||
Boldface indicates previously identified components of ODV.
*
Includes sequences determined by using MS/MS; see Tables 4 and 5 for a full description.