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. 2007 May 9;8:116. doi: 10.1186/1471-2164-8-116

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Copyright © 2007 Settles et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Informatic analysis of UniformMu FSTs validated by PCR. (A) Distribution of Mu insertion sites relative to transcribed maize sequences. (B) Schematics of the locus-specific primer design as constrained by available sequences. Left and right specific primers were designed when genomic sequence was available on either side of the insertion site. Right specific primers were designed when the insertion site defined novel maize genomic sequence. A presumptive right primer was designed for FSTs without a MuTIR sequence.