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. 2000 Oct 17;97(23):12457–12462. doi: 10.1073/pnas.220421797

Figure 4.

Figure 4

(A) CD100 stimulates the interaction between plexin-B1 and RacL61. VSV epitope-tagged plexin-B1 or plexin-A1 (0.5 μg) was cotransfected with myc-Rac (0.2 μg) and/or semaphorin (0.5 μg) in HEK293 cells as indicated. Cells were lysed and immunoprecipitated with anti-myc antibody. The immunoprecipitated samples were probed with anti-VSV antibody. Expression of Rac (Bottom, detected by anti-myc) and plexin (second panel from bottom, detected by anti-VSV) are shown. VSV-Plexin-B1 coimmunoprecipitated by myc-Rac is shown in the top two panels (for short and long exposure). Cotransfection of CD100 and Sema3A is indicated. Lanes 8 and 9 also were cotransfected with neuropilin-1 (0.5 μg). (B) A proposed model for Rac-GTP in CD100-stimulated plexin-B1 signaling. The ability of Rac-GTP to bind the plexinB1 receptor is enhanced on stimulation with CD100. Active Rac binds the receptor CRIB motif (denoted as a filled circle) and is predicted to affect actin reorganization.