Table 1. Quantification of the Ran-independent release of HBV genomes from mature virus-derived capsids.

Sample	% Genomes	% Protein
No substrate (D)	0	0
Substrate + BSA + ATP + GTP (C)	0	0
Substrate + reticulocyte lysate (B)	100	100
Substrate + BSA + ATP + GTP + Impα + Impβ (E)	28	0
Substrate + BSA + ATP + GTP + Impα + Impβ + Ran GDP (A)	40	40
Substrate + BSA + ATP + GTP + Impα + Impβ + Ran GDP + GTP[γS] (F)	28	6

In 50 cells per sample, the intranuclear hybridization signal was analyzed. The background, revealed from the sample in which no substrate was added, was subtracted and taken as 0%. The positive control, in which rabbit reticulocyte lysate was added, was taken as the 100% value. The letters in parentheses refer to the panels in Fig. 5. % Genomes, percentage of released intranuclear HBV genomes. % Protein, percentage of intranuclear control protein.