Figure 1.

A PKC inhibitor suppresses transcriptional activation of the ARE. HepG2 cells were stably transfected with the rat QR ARE-CAT reporter construct. After incubation in dimethyl sulfoxide (solvent control), tBHQ (100 mM), or PMA (100 nM) for 18 h, in the presence or absence of Ro-32–0432 (0.2 mM), cells were harvested, and cell lysates were ssayed for CAT activity. Quantitation of the results was performed by a phosphor imager; the level of CAT activity from solvent-treated cells was set at 1. The data shown are means of three independent experiments.