Figure 1.

Spectral analysis of the reaction of selenite with DHLA. Spectra were recorded after addition of 10 μl of a reaction mixture containing DHLA and selenite in an acidic solution of 50% EtOH (Materials and Methods) to 1 ml of 100% EtOH. Ratios given for each spectrum represent the molar ratio of free sulfhydryl to selenium in the reaction mixture.