Reduction of LNSe by bovine LD. Reaction mixtures contained 0.1 M
sodium phosphate buffer (pH 6.5), 1.0 mM NADPH, 300 μM LNSe, and 33
ng LD. After removal of an initial aliquot before addition of enzyme,
samples were taken at 1-min intervals up to 4 min. The area of the peak
representing LNSe determined for each time point by HPLC analysis was
converted to a percentage of the initial LNSe peak. The area units
given are the average of at least three independent experiments, and
the error bars represent one standard deviation from the mean.