Table 2. Summary of characteristics of the selected panel of 27 putative P. falciparum antigens.
|
Peptides∥
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Antigen | Protein* | Amino acids† | Percent sequence coverage (PfSpz) | Sporozoite specific‡ | Fraction | SP§ | TM¶ | Stringent criteria | Relaxed criteria | MudPIT runs |
| 1 | PF10_0179** | 1,904 | 0.6 | Yes | Soluble | 0 | 0 | 1 | 1 | 1 |
| 2 | PFL0800c | 208 | 49.5 | Yes | Soluble and insoluble | 1 | 0 | 14 | 16 | 6 |
| 3 | PFI0165c | 2,404 | 1.3 | No | Soluble and insoluble | 0 | 0 | 8 | 11 | 2 |
| 4 | PFC0210c | 396 | 10.1 | Yes | Soluble | 1 | 1 | 2 | 2 | 2 |
| 5 | PF14_0372 | 400 | 3.0 | No | Soluble and insoluble | 1 | 2 | 4 | 6 | 4 |
| 6 | PF11_0341 | 1,062 | 5.5 | No | Soluble | 0 | 1 | 0 | 6 | 3 |
| 7 | mal4T2c4.plt1 | 104 | NA | ? | Soluble | - | - | ? | ? | 0 |
| 8 | PF13_0278 | 954 | 1.6 | No | Soluble | 0 | 0 | 2 | 5 | 1 |
| 9 | PFE0765w | 2,133 | 0.7 | No | Soluble | 0 | 0 | 1 | 2 | 1 |
| 10 | PF11_0479 | 3,029 | 2.4 | No | Soluble and insoluble | 0 | 0 | 4 | 8 | 4 |
| 11 | PFC0450w | 109 | 16.5 | Yes | Soluble | 1 | 2 | 0 | 1 | 1 |
| 12 | PF14_0074 | 395 | 26.6 | No | Soluble | 0 | 0 | 8 | 8 | 3 |
| 13 | PFC0700c | 307 | NA | ? | Soluble | 0 | 1 | 0 | 0 | 0 |
| 14 | PF14_0291 | 1,234 | 1.0 | Yes | Insoluble | 0 | 0 | 0 | 1 | 1 |
| 15 | MAL8P1.78 | 100 | 48.8 | Yes | Soluble and insoluble | 0 | 0 | 9 | 9 | 4 |
| 16 | MAL13P1.218 | 253 | 3.4 | Yes | Soluble | 0 | 0 | 0 | 1 | 1 |
| 17 | PFD0425w | 984 | 25.3 | Yes | Soluble | 1 | 0 | 15 | 18 | 4 |
| 18 | PF13_0320 | 1,791 | 1.4 | No | Soluble and insoluble | 0 | 21 | 3 | 6 | 3 |
| 19 | PF11_0435 | 1,815 | 0.8 | Yes | Soluble | 0 | 6 | 1 | 1 | 1 |
| 20 | PFI0260 | 4,544 | 2.2 | No | Soluble and insoluble | 0 | 0 | 9 | 13 | 6 |
| 21 | PF11_0226 | 2,024 | 0.8 | No | Soluble and insoluble | 1 | 0 | 3 | 5 | 1 |
| 22 | PFA0515w | 1,471 | 3.3 | No | Soluble | 0 | 0 | 1 | 8 | 2 |
| 23 | MAL6P1.252 | 2,647 | 1.7 | No | Soluble | 0 | 1 | 3 | 3 | 1 |
| 24 | PF14_0236 | 1,781 | 3.4 | No | Soluble and insoluble | 0 | 0 | 2 | 6 | 3 |
| 25 | PF14_0751 | 188 | 2.1 | Yes | Soluble | 1 | 1 | 0 | 1 | 1 |
| 26 | PF14_0648 | 96 | 0.5 | No | Soluble | 0 | 0 | 2 | 2 | 1 |
| 27 | PF11_0456 | 1,464 | 4.0 | No | Soluble | 0 | 0 | 0 | 5 | 2 |
| CSP | PFC0210c | 397 | 10.1 | Yes | Soluble | 1 | 1 | 2 | ||
| SSP2 | PF13_0201 | 574 | 36.1 | Yes | Soluble | 1 | 0 | 24 | ||
| EXP-1 | PF11_0224 | 162 | 72.9 | No | Soluble and insoluble | 1 | 1 | 6 | ||
| LSA-1 | X56203 | 1,909 | NA | No | NA | 1 | 1 | NA | ||
NA, not applicable.
Gene accession number.
The number of amino acids in the translated gene product of initial gene loci predictions, based on an incomplete and heterogeneous P. falciparum genomic sequence database (09/05/01 version).
“Yes” indicates sequences found only in sporozoite preparations, and “No” indicates sequences found in both the sporozoite preparations and preparations from different parasite life stages.
Absence (0) or presence (1) of predicted N-terminal signal peptide (SP), based on the SignalP algorithm (see http://plasmodb.org).
Absence (0) or presence (1-21) of predicted transmembrane domains (TM), based on the TMHMM algorithm (see http://plasmodb.org).
The number of peptides identified by using either the stringent or relaxed criteria, respectively, as described in the text.
Sequences can be accessed via PlasmoDB, the official database of the malaria parasite genome project, at (http://plasmodb.org).