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Inhibition of bradykinin binding by randomized peptide libraries with defined residues at given positions. The effect of defined residues at position 1 (A), position 5 (B), and position 9 (C) of nonameric peptide libraries are shown. All peptides were tested at 1.0 μM in the presence of 0.1 μM [3H]bradykinin. Amino acids are indicated in single-letter code. The OppA concentration was 20 μg/ml; error bars indicate the standard deviation (Xσn − 1), n = 3.
