Table 1. Time course of insoluble tau accumulation.

Time	Thrashes per min, mean ± SEM	RIPA tau, percent of total	FA tau, percent of total
Day 1	27.6 ± 3.3	0.8	0.09
Day 3	11.9 ± 2.3	9.8	4.2
Day 5	4.6 ± 1.4	5.0	10.6

Developmentally staged 337^M-1/glp-4 (bn2) animals were grown for 1, 3, and 5 days after hatching at 25°C. Thrashing rates are means from 10 animals. Tau in RAB, RIPA, and FA fractions were analyzed by quantitative immunoblotting using antibody 17026. Total tau is the sum of all fractions.