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(A) Gas chromatography-chemical ionization MS with selected ion monitoring analysis at m/z 283 for N-benzoylaminoketone (15). Nicotine, P450 2A6, and cofactors were incubated for 70 min and analyzed by Method 1, as described in Materials and Methods. The peak has the correct retention time for 15. (B) Electron impact-MS of standard 15. (C) 15 isolated from metabolism of nicotine by P450 2A6.
