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. 2000 Oct 24;97(23):12498–12502. doi: 10.1073/pnas.220425497

Figure 5.

Figure 5

Vigilin binding selectively inhibits PMR-1 cleavage of vitellogenin mRNA 3′-UTR; 500 fmol of uniformly labeled albumin mRNA (Alb) or vitellogenin mRNA 3′-UTR (Vit) were incubated with buffer alone (No protein), 40 units of PMR-1 (PMR-1), 500 ng of recombinant vigilin (Vigilin), or PMR-1 plus vigilin (PMR-1 and Vigilin). Samples recovered from each reaction were electrophoresed on a 6% polyacrylamide-urea gel and visualized by PhosphorImager. Lane 1 contains a size marker of HinfI-cut φX174 DNA.