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. 2000 Nov 7;97(23):12513–12518. doi: 10.1073/pnas.97.23.12513

Figure 2.

Figure 2

(Left) Western analysis of p53 pathway. HeLa cell proteins were harvested at the indicated hours after E2 infection or mock-infection, electrophoresed, transferred, and probed with antibodies specific for p53, p21, or human mdm2, as indicated. (Right) Northern analysis of p53 pathway. HeLa cell RNA was isolated at the indicated hours after E2 infection or mock-infection. After electrophoresis and transfer, p53, p21, and mdm2 mRNA was detected by hybridization to the appropriate radiolabeled cDNA probe.