Figure 4.

(Left) Western analysis of retinoblastoma family members. HeLa cell protein was prepared at the indicated hours after E2 infection or mock-infection. After electrophoresis and transfer, specific antibodies were used to detect HPV18 E7, p105^Rb, p107, and p130. The hyperphosphorylated (p) and hypophosphorylated (o) form of p105^Rb are indicated. (Right) Northern analysis of retinoblastoma family members. HeLa cell RNA was prepared at the indicated hours after mock-infection or E2 infection. After electrophoresis and transfer, p105^Rb, p107, and p130 mRNA were detected by hybridization to the appropriate radiolabeled cDNA probe.