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Transactivation of a C/EBPα promoter-reporter gene by Sp3 and AP-2α. Cells were cotransfected either with C/EBPα promoter-reporter or promoterless genes with or without Sp3 and/or AP-2α expression vectors. Cell lysates were prepared 48 h after transfection and assayed for luciferase activity. (A) Transfection into 3T3-L1 preadipocytes or (B) transfection into Schneider SL2
