Kinetics of expression of CUP/AP2, C/EBPα, and Sp3
after induction of adipocyte differentiation. 3T3-L1 preadipocytes were
subjected to the differentiation protocol. Nuclear extracts were
prepared at the times indicated and subjected to immunoblotting with
antibody directed against either (A) C/EBPα,
(B) AP-2α, or (C) Sp3. The previously
published data shown in A and B
were used for comparison of the kinetics of the expression of
C/EBPα and AP-2α, respectively, with those of Sp3
(C). [A is modified with permission from
ref. 10 (Copyright 1997, National Academy of Sciences, U.S.A.);
B is modified with permission from ref. 7 (Copyright
1998, National Academy of Sciences, U.S.A.)].