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. 2000 Oct 24;97(23):12524–12529. doi: 10.1073/pnas.220430297

Figure 3.

Figure 3

Effects of hsp90 deletions on p23 binding. (A) GST-1–728 and GST-1–698 were compared for p23 binding in the presence or absence of 2 mM ATP. Background measurements were assessed by omitting p23 or the p23 antibody JJ3. (B) hsp90, GST-1–728, and GST-1–573 were assessed for p23 binding in the presence or absence of ATP, adenosine 5′-diphosphate, or geldanamycin (GA). (C) The GST-hsp90 constructs indicated were compared for p23 binding in the presence or absence of ATP. In all cases, the hsp90 constructs from p23 complexes were resolved by SDS/PAGE and stained with Coomassie Blue.