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Binding of hsp90 mutants to p23. (A) GST-206–728 and GST-287–728 were assessed for p23 binding in the presence or absence of ATP. GST-1–728 was included as a positive control and the total protein assayed for each mutant is shown in lanes labeled (*) (B) p23 Binding is shown using hsp90 or the two full-length hsp90 mutants, R362/3A and L392S. The total protein assayed is shown in the last three lanes.
