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. 2000 Oct 24;97(23):12524–12529. doi: 10.1073/pnas.220430297

Figure 6.

Figure 6

Dimer-dependent binding of p23. (A) SDS/PAGE analysis of purified 1–573 and FKBP-1–573 proteins. (B) Dimerization of FKBP-1–573 is dependent on the proportion of added bivalent drug. FKBP-1–573 was incubated for 1 h at 30° in the presence of the bivalent drug AP20187 (closely related to AP1903; ref. 38). drug/FKBP-1–573 ratios were varied between 0 and 2, and monomers (M) and dimers (D) were resolved by native PAGE. (C) p23 Binding correlates with drug-induced dimerization. Samples were prepared with FKBP-1–573 plus various ratios of AP20187 as in B and tested for p23-binding activity. Shown is the amount of FKBP-1–573 bound to p23 and resolved by SDS/PAGE and Western blotting with an antibody to hsp90.