Table 1.
Characteristics of purified AcsAB before and after Ni activation
| Batch | 1 | 2 | 3 |
|---|---|---|---|
| Mass of cell paste (g) | 70 | 65 | 80 |
| Ni content before activation (Ni/αβ) | 1.5 | 1.6 | 1.0 |
| Fe content before activation (Fe/αβ) | 16 | 22 | 14 |
| CO oxidation activity before/after activation (units/mg) | 160/140 | 200(220)/250 | 100(90)/100 |
| CO exchange activity before/after activation (units/mg) | 0/0.06 | 0/0.15 | 0/0.25 |
| NiFeC Signal intensity before/after activation (spin/αβ) | 0/0.15 | 0/0.38 | 0/0.15 |
| gav = 1.94 Signal intensity before/after activation (spin/αβ) | 1.12/0.75 | 0.95(0.55)/0.93 | 0.83(0.66)/0.82 |
| gav = 1.82 Signal intensity before activation (spin/αβ) | 0.29 | 0.31 | 0.25 |
| gav = 1.86 Signal intensity before/after activation (spin/αβ) | 0.21/0.24 | 0.13/0.18 | 0.08/0.09 |
Batch 1 was activated with Ni under 1 atm Ar, while batches 2 and 3 were activated under 1 atm CO. Values in parentheses for batches 2 and 3 are from samples incubated overnight in the absence of reductants and under 1 atm Ar.