Skip to main content
. 2000 Oct 24;97(23):12536–12540. doi: 10.1073/pnas.220427297

Figure 1.

Figure 1

Interaction of endogenous hER with AIB1 is demonstrated by coimmunoprecipitation. MCF-7 cells were treated with E2 for 0.5 h and extracts prepared as described in Materials and Methods (CE, lane 1, NEs, lane 2, or NEv, lane 3). Equal volumes of extract per packed cell volume were loaded to allow direct visual comparison of lanes. Immunoprecipitation of NEs (lane 4) or NEv (lane 5) extracts by using anti-ER antibody was performed and analyzed by Western blot. (A) Blots were cut in half horizontally along the 97,000 marker. The top half was immunostained for AIB1(Mr, 160,000), and the bottom half for hER (Mr, 66,000). Nonspecific binding of NEs (lane 6) and NEv (lane 7) extracts is shown by using anti-IgG immunoprecipitation. The original extracts (lanes 2 and 3) represent 15% of the input into immunoprecipitation reactions. (B) Experiment conducted as in A, except the top half was immunostained for SRC-1 (Mr, 160,000). The original extracts (lanes 2 and 3) represent 13% of the input into immunoprecipitation reactions.