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Affinity of mER binding to the GST-AIB1 fusion protein. A constant limiting amount of GST-AIB1 immobilized on glutathione beads was incubated with increasing concentrations of mER. Beads were washed and bound mER detected by Western blot as described in Materials and Methods. The intensity of the ER bands was analyzed and graphed as a binding curve as a function of mER concentration.
