Figure 2.

Mutant SOD accumulates in intracellular inclusion bodies. (A) HEK cells were transfected wild-type or mutant SOD as indicated. Cells on the Right (c, d, g, h, k, and l) were treated overnight with lactacystin, followed by examination by indirect immunofluorescence with an affinity-purified antibody to SOD (α-SOD; a, c, e, g, i, and k) and a mAb to the HA epitope tag (α-SOD; b, d, f, h, j, and l). (Bar = 15 μm.) (B) Cells transfected and treated as in A were counted and scored for the presence of inclusion bodies. Between 200 and 430 cells were counted for each condition. Single (P < 0.05) and double (P < 0.001) asterisks indicate statistical significance relative to untreated SOD based on Student's t test.