Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1988 Feb;130(2):244–251.

AIDS-related Kaposi's sarcoma displays differential expression of endothelial surface antigens.

P A Scully 1, H K Steinman 1, C Kennedy 1, K Trueblood 1, D M Frisman 1, J R Voland 1
PMCID: PMC1880519  PMID: 3341448

Abstract

The authors studied 11 cases of Kaposi's sarcoma (KS) in patients with the acquired immunodeficiency syndrome (AIDS) for their reactivity with two monoclonal antibodies (B721 and E431) that recognize endothelial cell surface antigens. Reactivity of these antibodies with KS was compared with the reactivity of other known endothelial markers (F8rAg, Ia, HCL-1). Staining was done with avidin-biotin-alkaline phosphatase immunohistochemistry on acetone-fixed frozen sections. In all samples of tumor both the spindle cell component and the vascular lining cells stained with both B721 and E431. In general, the spindle cells stained less intensely than did the vascular lining cells. There was both intratumor and intertumor variability. B721 and E431 are proposed as two additional markers for KS, and it is suggested that their reactivity with the tumor supports the hypothesis that KS is derived from vascular endothelium. The possibility is also raised that the variability of staining for vascular markers could have diagnostic possibilities, and further studies for investigation of this hypothesis are suggested.

Full text

PDF
244

Images in this article

247Figure 1
on p.247
249Figure 3
on p.249
248Figure 2
on p.248

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akhtar M., Bunuan H., Ali M. A., Godwin J. T. Kaposi's sarcoma in renal transplant recipients. Ultrastructural and immunoperoxidase study of four cases. Cancer. 1984 Jan 15;53(2):258–266. doi: 10.1002/1097-0142(19840115)53:2<258::AID-CNCR2820530213>3.0.CO;2-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnstable C. J., Bodmer W. F., Brown G., Galfre G., Milstein C., Williams A. F., Ziegler A. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 1978 May;14(1):9–20. doi: 10.1016/0092-8674(78)90296-9. [DOI] [PubMed] [Google Scholar]
  3. Beckstead J. H., Wood G. S., Fletcher V. Evidence for the origin of Kaposi's sarcoma from lymphatic endothelium. Am J Pathol. 1985 May;119(2):294–300. [PMC free article] [PubMed] [Google Scholar]
  4. Doellgast G. J., Meis P. J. Use of specific inhibitors to disciminate alkaline phosphatase isoenzymes originating from human liver, placenta and intestine: absence of meconial alkaline phosphatase in maternal serum. Clin Chem. 1979 Jul;25(7):1230–1233. [PubMed] [Google Scholar]
  5. Dorfman R. F. Kaposi's sarcoma revisited. Hum Pathol. 1984 Nov;15(11):1013–1017. doi: 10.1016/s0046-8177(84)80243-9. [DOI] [PubMed] [Google Scholar]
  6. Emmott R. C., Javadpour N., Bergman S. M., Soares T. Correlation of the cell surface antigens with stage and grade in cancer of the bladder. J Urol. 1979 Jan;121(1):37–39. doi: 10.1016/s0022-5347(17)56650-1. [DOI] [PubMed] [Google Scholar]
  7. Flotte T. J., Hatcher V. A., Friedman-Kien A. E. Factor VIII-related antigen in Kaposi's sarcoma in young homosexual men. Arch Dermatol. 1984 Feb;120(2):180–182. [PubMed] [Google Scholar]
  8. Guarda L. G., Silva E. G., Ordóez N. G., Smith J. L., Jr Factor VIII in Kaposi's sarcoma. Am J Clin Pathol. 1981 Aug;76(2):197–200. doi: 10.1093/ajcp/76.2.197. [DOI] [PubMed] [Google Scholar]
  9. Gupta S., Safai B. Deficient autologous mixed lymphocyte reaction in Kaposi's sarcoma associated with deficiency of Leu-3+ responder T cells. J Clin Invest. 1983 Feb;71(2):296–300. doi: 10.1172/JCI110769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirschberg H., Moen T., Throsby E. Specific destruction of human endothelial cell monolayers by anti-DRw antisera. Transplantation. 1979 Aug;28(2):116–120. doi: 10.1097/00007890-197908000-00009. [DOI] [PubMed] [Google Scholar]
  11. Knowles D. M., 2nd, Tolidjian B., Marboe C., D'Agati V., Grimes M., Chess L. Monoclonal anti-human monocyte antibodies OKM1 and OKM5 possess distinctive tissue distributions including differential reactivity with vascular endothelium. J Immunol. 1984 May;132(5):2170–2173. [PubMed] [Google Scholar]
  12. Lampson L. A., Levy R. Two populations of Ia-like molecules on a human B cell line. J Immunol. 1980 Jul;125(1):293–299. [PubMed] [Google Scholar]
  13. Mason D. Y., Sammons R. Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents. J Clin Pathol. 1978 May;31(5):454–460. doi: 10.1136/jcp.31.5.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Modlin R. L., Hofman F. M., Kempf R. A., Taylor C. R., Conant M. A., Rea T. H. Kaposi's sarcoma in homosexual men: an immunohistochemical study. J Am Acad Dermatol. 1983 May;8(5):620–627. doi: 10.1016/s0190-9622(83)70067-8. [DOI] [PubMed] [Google Scholar]
  15. Nadji M., Morales A. R., Ziegles-Weissman J., Penneys N. S. Kaposi's sarcoma: immunohistologic evidence for an endothelial origin. Arch Pathol Lab Med. 1981 May;105(5):274–275. [PubMed] [Google Scholar]
  16. Posnett D. N., Marboe C. C., Knowles D. M., 2nd, Jaffe E. A., Kunkel H. G. A membrane antigen (HC1) selectively present on hairy cell leukemia cells, endothelial cells, and epidermal basal cells. J Immunol. 1984 Jun;132(6):2700–2702. [PubMed] [Google Scholar]
  17. Reichert C. M., O'Leary T. J., Levens D. L., Simrell C. R., Macher A. M. Autopsy pathology in the acquired immune deficiency syndrome. Am J Pathol. 1983 Sep;112(3):357–382. [PMC free article] [PubMed] [Google Scholar]
  18. Rutgers J. L., Wieczorek R., Bonetti F., Kaplan K. L., Posnett D. N., Friedman-Kien A. E., Knowles D. M., 2nd The expression of endothelial cell surface antigens by AIDS-associated Kaposi's sarcoma. Evidence for a vascular endothelial cell origin. Am J Pathol. 1986 Mar;122(3):493–499. [PMC free article] [PubMed] [Google Scholar]
  19. Slovin S. F., Frisman D. M., Tsoukas C. D., Royston I., Baird S. M., Wormsley S. B., Carson D. A., Vaughan J. H. Membrane antigen on Epstein--Barr virus-infected human B cells recognized by a monoclonal antibody. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2649–2653. doi: 10.1073/pnas.79.8.2649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Small C. B., Klein R. S., Friedland G. H., Moll B., Emeson E. E., Spigland I. Community-acquired opportunistic infections and defective cellular immunity in heterosexual drug abusers and homosexual men. Am J Med. 1983 Mar;74(3):433–441. doi: 10.1016/0002-9343(83)90970-1. [DOI] [PubMed] [Google Scholar]
  21. Turner R. R., Beckstead J. H., Warnke R. A., Wood G. S. Endothelial cell phenotypic diversity. In situ demonstration of immunologic and enzymatic heterogeneity that correlates with specific morphologic subtypes. Am J Clin Pathol. 1987 May;87(5):569–575. doi: 10.1093/ajcp/87.5.569. [DOI] [PubMed] [Google Scholar]
  22. Voland J. R., Frisman D. M., Baird S. M. Presence of an endothelial antigen on the syncytiotrophoblast of human chorionic villi: detection by a monoclonal antibody. Am J Reprod Immunol Microbiol. 1986 May;11(1):24–30. doi: 10.1111/j.1600-0897.1986.tb00023.x. [DOI] [PubMed] [Google Scholar]
  23. Yam L. T., English M. C., Janckila A. J., Ziesmer S., Li C. Y. Immunocytochemical characterization of human blood cells. Am J Clin Pathol. 1983 Sep;80(3):314–321. doi: 10.1093/ajcp/80.3.314. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES