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. 2000 Oct 24;97(23):12577–12582. doi: 10.1073/pnas.230435597

Figure 2.

Figure 2

Either NLS1APC or NLS2APC is sufficient to translocate the cytoplasmic protein βGal into the nucleus. βGal-fusion proteins were expressed in mouse L cells and detected by immunofluorescence microscopy. Nuclei were visualized with 4′,6-diamidino-2-phenylindole (DAPI). Areas of overlap between the βGal fusion protein (green) and the nuclei (blue) appear an aqua color. (Bar = 10 μm.) For each construct, 100 cells were scored for βGal localization with conventional fluorescence microscopy. The results of four independent experiments are presented as the incidence of nuclear βGal staining ± SD as follows: βGal, 27(±6)%; βGal-NLSSV40 T-ag, 100(±0)%; βGal-NLS1APC, 73(±11)%; βGal-mNLS1APC, 30(±13)%; βGal-NLS2APC, 69(±8)%; βGal-mNLS2APC, 35(±4)%; βGal-NLS1,2APC, 83(±6)%; and βGal-(NLS2APC)2, 91(±4)%.