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Yeast two-hybrid interactions between GRASP65 (amino acids 7–120) and Plk. Conserved polo-boxes (1–3, left to right) are indicated by darkly shaded boxes. Binding interaction between Plk and GRASP65 was determined by growth on selective medium and β-galactosidase activity. Data are not available for the full-length and 323–603 constructs because of background β-galactosidase activity in bait strains.
