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. 2000 Oct 24;97(23):12589–12594. doi: 10.1073/pnas.220423497

Figure 3.

Figure 3

GRASP65 coimmunoprecipitates with Plk. (A) Anti-FLAG immunoprecipitations (IP). HA-Plk is indicated by arrow. (B) Anti-HA immunoprecipitations. GRASP65-FLAG is indicated by arrow. Fusion proteins were detected by immunoblotting (IB) with their respective epitope antibodies. Cos-7 cells were transfected with vector (pCIneo) only (lane 1) or cotransfected with GRASP65-FLAG and HA-Plk WT (lane 2), KD (lane 3), polo-box 1 (a.a. 411–439) FS (lane 4), or T210D (lane 5) constructs at a 1:3 ratio.