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. 2007 May 30;104(25):10619–10624. doi: 10.1073/pnas.0701421104

Fig. 1.

Fig. 1.

Bone mass and bone formation are dramatically increased in adult PYK2−/− mice at 18 weeks of age. (A) Radiography of isolated femurs from female WT control and PYK2−/− mice. (B and C) μCT images of the distal femur (B) and second lumbar vertebrae (C) of WT control and PYK2−/− mice. (D–G) Quantitative analysis of μCT images of the distal femur and second lumbar vertebrae from WT control mice and PYK2−/− mice. (D) Volumetric bone mineral density (vBMD) in WT control (hatched bars) and PYK2−/− mice (black bars). (E) Trabecular number (Tb.N). (F) Trabecular thickness (Tb.Th). (G) Cancellous bone volume per tissue volume. (H) Calcein labeling of cancellous bone in proximal tibia (green) was visualized by fluorescence micrography, with Villanueva bone counterstain (red background). (Scale bar: 100 μm.) (I–L) Dynamic histomorphometry of cancellous bone. Bone surface referent bone formation rate (BFR/BS) (I), mineralizing surface per bone surface (MS/BS) (J), and mineral apposition rate (K) are significantly increased in PYK2−/− animals. (L) Osteoclast surface (Oc.S/BS). (M) Serum CTX concentration. All data are presented as means ± SEM; n = 12–14 animals per genotype. ∗, P < 0.01; #, P < 0.05. Significance was determined by ANOVA followed by Fisher's protected least significant difference test to compare differences among groups. P < 0.05 was considered significant.