Figure 5.
Real-time RT-PCR analysis in BMM after the addition of 100 ng/ml ricin for 5 hrs, in the presence or absence of a 30 min pretreatment with 25 μM zVAD.fmk. The results were expressed as fold induction, using glyceraldehyde phosphate dehydrogenase (GAPDH) as invariant comparator. The experiment was repeated three times, with similar results. Each treatment was performed on triplicate culture dishes. Data are displayed as mean ± standard error of the mean. Asterisks above bars show comparison between treatments and controls; P values displayed below bars of ricin plus zVAD.fmk treatment show the comparison of this treatment to treatment by ricin alone. NS, not significantly different; *, p<0.05; **, p<0.01; ***, p<0.001.