TABLE 1.
Assignment of MALDI-TOF-MS peaks found in gel slice 192
This list of matched peptides, using our novel peptide matching algorithm, corresponds to those peptides matched for slice 192 (Fig. 1B). Accuracy is comparable with all gel slices in this experiment.
| Theoretical peptidea | Proteinb | Positionsc | Modificationd | Theoretical masse | Actual massf | Differenceg | Abundanceh | Notesi |
|---|---|---|---|---|---|---|---|---|
| aa | Da | ppm | % BP | |||||
| ITKPGSIDSNNQLFAPGGR | eIF4G | 221–239 | 1972.0246 | 1972.0124 | 6 | 11.66 | ||
| IAHFLDR | eIF3e | 10–16 | 871.4784 | 871.4886 | −12 | 9.85 | ||
| NLYSDDIPHALR | eIF3e | 60–71 | 1413.7121 | 1413.7102 | 1 | 29.73 | ||
| QEYLDTLYR | eIF3e | 125–133 | 1200.5895 | 1200.5955 | −5 | 7.56 | ||
| FQYECGNYSGAAEYLYFFR | eIF3e | 137–155 | Acr: 141 | 2399.0437 | 2399.1377 | −39 | 9.13 | |
| VLVPATDR | eIF3e | 156–163 | 870.5043 | 870.4962 | 9 | 9.51 | ||
| LASEILMQNWDAAMEDLTR | eIF3e | 173–191 | MSO: 179, 186 | 2239.0369 | 2239.1224 | −38 | 67.7 | Trypsin |
| ETIDNNSVSSPLQSLQQR | eIF3e | 194–211 | 2015.9992 | 2015.9832 | 8 | 15 | ||
| LFIFETFCR | eIF3e | 338–346 | Acr: 345 | 1246.6288 | 1246.6375 | −7 | 6.73 | |
| AEEQMLK | eIF3a | 818–824 | MSO: 822 | 864.4131 | 864.4868 | −85 | 9.99 | |
| IGVDLIMK | eIF3f | 247–254 | MSO: 253 | 904.5172 | 904.4695 | 53 | 39.95 | Calibrant |
| DTLGPMQK | eIF3g | 173–180 | MSO: 178 | 905.4397 | 905.4725 | −36 | 6.01 | |
| TAQGSLSLK | eIF3h | 157–165 | 904.5098 | 904.4695 | 45 | 39.95 | Calibrant | |
Amino acid sequence of the matched peptide.
Protein from which the matched peptide is derived. eIF4G refers to recombinant S-eIF4G-(1015–1118) used in this study.
Amino acid position of the theoretical peptide in the corresponding protein. Amino acid positions for eIF4G correspond to the recombinant protein.
Oxidation of methionine (MSO) or acrylamidation of cysteine (Acr) residues are accounted for in theoretical mass. Amino acid position of modified residues in the matched peptide is indicated.
The mass of the theoretical peptide (in Da) was generated by the Peptide Mass tool at the ExPASy Proteomics site on line.
Mass of an actual peak generated by MALDI-TOF-MS (in Da) that is matched to a particular theoretical peptide.
Calculated difference in theoretical and actual masses expressed as parts/million.
Height of the peak as compared with the most abundant peak in a particular spectrum generated by MALDI-TOF-MS.
Matched peptides that are also within 100 ppm of either trypsin autodigestion or internal calibrant peaks are indicated.