Skip to main content
. 2000 Oct 31;97(23):12619–12624. doi: 10.1073/pnas.230224097

Figure 6.

Figure 6

Raising the level of TFIIIC2 stimulates pol III transcription in ovarian epithelial cell extracts. (A) In vitro transcription by using 10 μg of ROSE 199 ovarian cell extract and 250 ng of pVA1 template. Reactions 2, 3, and 4 were supplemented with 2, 4, and 8 μl of phosphocellulose B, respectively. Reactions 6, 7, and 8 were supplemented with 2, 4, and 8 μl of phosphocellulose C, respectively. (B) In vitro transcription assay by using 10 μg of ROSE 199 ovarian cell extract and 250 ng of pVA1 template. Reactions 2, 3, and 4 were supplemented with 3, 6, and 9 μl, respectively, of TFIIIC that had been immunopurified by using antiserum 4286 against TFIIIC110. Reactions 5, 6, and 7 were supplemented with 3, 6, and 9 μl, respectively, of material that had been mock immunopurified by using preimmune serum.